Dipyridamole inhibits platelet aggregation induced by oxygen-derived free radicals.
نویسندگان
چکیده
Pyrogallol (a generator of superoxide anions) caused 50% increase in platelet aggregation induced by 400 microM of arachidonic acid. Dipyridamole did not produce a statistically significant inhibition of arachidonic-acid induced platelet aggregation, but it caused 100% inhibition of pyrogallol-stimulated platelet aggregation. Ferrous salts (Fe2+) induced 34% platelet aggregation which was inhibited (79.6%) by a concentration of dipyridamole of 10 microM. Dipyridamole inhibited ferrous-induced lipid peroxidation with IC-50 values of 17.5 microM. When arachidonic acid was used as aggregating agent, the corresponding IC-50 value was 140.5 microM. These results indicate that dipyridamole prevented platelet activation induced by oxygen-derived free radicals.
منابع مشابه
Antiplatelet effect of the pyrimido-pyrimidinic derivative RA-642.
Amongst pyrimido-pyrimidine derivatives (p-p), dipyridamole (Dip) acts as a coronary vasodilator and platelet aggregation inhibitor (l-3), mopidamole (Mop) has been used in association with antimetastatic drugs (4,5), and RA-642 is endowed with vasodilating and hypertensive effects (6-8). We also reported that RA-642 inhibited lipid peroxidation induced by oxygen free radicals (9). We have foun...
متن کاملDifferential effects of the pyrimido-pyrimidine derivatives, dipyridamole and mopidamol, on platelet and vascular cyclooxygenase activity.
The chronic administration of 10 mg/kg/day of dipyridamole to rats produced 33.7% inhibition of platelet aggregation induced with ADP and a 93% increase in 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) in vascular samples, versus saline-treated rats. Mopidamol, 8.3 mg/kg/day, caused 50.6% inhibition of ADP-induced platelet aggregation, 37.6% inhibition of aggregation induced with arachidoni...
متن کاملMechanisms involved in adenosine triphosphate--induced platelet aggregation in whole blood.
OBJECTIVE Effects on platelet aggregation of adenosine triphosphate (ATP) released from damaged cells and from platelets undergoing exocytosis have not been clearly established. In this study we report on the effects of ATP on platelet aggregation in whole blood. METHODS AND RESULTS Aggregation, measured using a platelet-counting technique, occurred in response to ATP and was maximal at 10 to...
متن کاملAdenosine derived from ADP can contribute to inhibition of platelet aggregation in the presence of a P2Y12 antagonist.
OBJECTIVE To investigate whether adenosine diphosphate (ADP)-derived adenosine might inhibit platelet aggregation, especially in the presence of a P2Y₁₂ antagonist, where the effects of ADP at the P2Y₁₂ receptor would be prevented. METHODS AND RESULTS Platelet aggregation was measured in response to thrombin receptor activator peptide by platelet counting in platelet-rich plasma (PRP) and who...
متن کاملRole of oxygen derived free radicals in platelet activating factor induced bowel necrosis.
The mechanism of tissue and cell injury in ischaemic bowel necrosis is unclear. The present study investigated the role of oxygen derived free radicals in the development of bowel necrosis using injections of platelet activating factor (PAF) into the mesenteric vasculature. Animals were pretreated with allopurinol or superoxide dismutase together with catalase, before administration of PAF. Sup...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Thrombosis research
دوره 66 4 شماره
صفحات -
تاریخ انتشار 1992